Novel Method to Isolate RNA from Live Cells in their Natural Microenvironment Found
A research has been carried out by the University of Pennsylvania in which study researchers found a way by which they can separate RNA from live cells. They have done so by keeping cells in their natural microenvironment only.
The researchers were of the view that through this way other cells would also not get affected. In order to know about gene expression in cells when they are in natural settings, it would be required that inner workings of cells can be known.
There are chances that each cell may appear to be same. But it shall however, be noted that every cell differs at molecular level. Study researchers wanted to know about the level of RNA that is present in single cell in intact tissue.
By knowing the above, the researchers were of the view that they would get to know about the functioning of mammalian cells as well as how their function gets spoiled due to an array of diseases.
In order to get RNA, a non-invasive method was found. Through Swiss-Army-Knife-type of molecule, was used to get mRNA from a cell. After passing through the stages of method, the study researchers were able to profile gene expression.
It was found that cells when present in suspension are able to express more genes in comparison to cells when present in intact tissue.
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